Abstract
Guinea pigs were immunized with glycoprotein gD-2t in SAF-m or saline, then challenged with herpes simplex virus, type 2 (HSV-2). Animals given gD-2t in SAF-m had higher anti-gD-2t antibodies, fewer and less severe vaginal lesions, and decreased ganglionic latency compared to animals given gD-2t in saline. Leucocytes from animals vaccinated with gD-2t in SAF-m had greater proliferative responses to gD-2t in vitro than cells from control animals. MHC II-restricted, gD-2t-specific cytotoxic T cells were induced in guinea pigs vaccinated with gD-2t in SAF-m. Thus, immunization of guinea pigs with gD-2t in SAF-m markedly reduced the incidence and severity of primary HSV-2 by eliciting both humoral and cell-mediated responses.
MeSH terms
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Acetylmuramyl-Alanyl-Isoglutamine / administration & dosage
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Acetylmuramyl-Alanyl-Isoglutamine / analogs & derivatives
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Adjuvants, Immunologic / administration & dosage
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Animals
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Antibodies, Viral / biosynthesis
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Cell Division / immunology
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Cytotoxicity, Immunologic
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Female
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Guinea Pigs
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Herpes Genitalis / prevention & control*
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Herpesviridae Infections / immunology
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Herpesviridae Infections / prevention & control*
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Herpesvirus 2, Human / immunology
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Immunity, Cellular
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Lymphocytes / immunology
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Polysorbates / administration & dosage
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Recombinant Proteins / administration & dosage
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Squalene / administration & dosage
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Squalene / analogs & derivatives
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Vaginal Diseases / immunology
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Vaginal Diseases / microbiology
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Vaginal Diseases / prevention & control*
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Viral Envelope Proteins / administration & dosage*
Substances
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Adjuvants, Immunologic
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Antibodies, Viral
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Polysorbates
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Recombinant Proteins
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Syntex adjuvant formulation
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Viral Envelope Proteins
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glycoprotein D-herpes simplex virus type 2
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Acetylmuramyl-Alanyl-Isoglutamine
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Squalene