Abstract
Induction of mouse glutathione S-transferase (GST) Ya gene expression by a variety of chemical agents is mediated by a regulatory element, EpRE, composed of an Ets and two adjacent AP-1-like binding sites. In this report we present evidence that the basal and inducible activity of EpRE is mediated by AP-1 transcription factor and that the cooperative interaction between AP-1 and an Ets protein contributes to enhance the EpRE inducibility. We also show that EpRE, similar to a single AP-1 site, when ligated to GST Ya gene promoter, is transactivated by c-Fos/c-Jun or c-Fos/Jun-B heterodimer and that c-Jun/c-Jun homodimer is an activator of an AP-1 site only in the context of collagenase gene promoter.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cells, Cultured
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DNA / metabolism*
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Enhancer Elements, Genetic
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Enzyme Induction
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Gene Expression*
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Glutathione Transferase / biosynthesis*
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Glutathione Transferase / genetics
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Mice
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Molecular Sequence Data
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Nuclear Proteins / isolation & purification
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Nuclear Proteins / metabolism
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Oligodeoxyribonucleotides
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Proto-Oncogene Proteins / isolation & purification
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-ets
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Proto-Oncogene Proteins c-jun / isolation & purification
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Proto-Oncogene Proteins c-jun / metabolism*
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Transcription Factors / metabolism
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Transfection
Substances
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Nuclear Proteins
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Oligodeoxyribonucleotides
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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Proto-Oncogene Proteins c-jun
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Transcription Factors
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DNA
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Glutathione Transferase