Abstract
Interferon-gamma (IFN-gamma) had been shown to increase superoxide production of cultured monocytes from patients with chronic granulomatous disease (CGD). To elucidate the mechanism of the IFN-gamma-induced improvement of superoxide production of cultured monocytes from patients with CGD we examined the influence of IFN-gamma on the expression and the activity of the NADPH-oxidase in the monocytic cell-line Mono Mac 6. After cultivation of Mono Mac 6-cells in the presence of 500 U/ml IFN-gamma the superoxide production was found to be increased as well as the expression of the p47-phox cytosolic protein of the phagocytic NADPH-oxidase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Cytosol / metabolism
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Genes, Recessive
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Granulomatous Disease, Chronic / genetics
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Granulomatous Disease, Chronic / immunology
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Granulomatous Disease, Chronic / metabolism*
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Humans
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Interferon-gamma / pharmacology*
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Monocytes / drug effects
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Monocytes / immunology
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Monocytes / metabolism
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NADPH Dehydrogenase / deficiency
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NADPH Dehydrogenase / metabolism*
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NADPH Oxidases
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Phagocytes / drug effects
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Phagocytes / immunology
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Phagocytes / metabolism*
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Phosphoproteins / deficiency
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Phosphoproteins / metabolism*
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Superoxides / metabolism
Substances
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Phosphoproteins
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Superoxides
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Interferon-gamma
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NADPH Oxidases
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neutrophil cytosolic factor 1
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NADPH Dehydrogenase