Abstract
An NlaIV polymorphism in the 5' untranslated region of the MDM2 gene is described. MDM2 was sublocalised by fluorescence in situ hybridisation with a yeast artificial chromosome probe to 12q14.3-12q15. We demonstrate the use of the polymorphism to assess allelic imbalance in breast tumours.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alleles
-
Base Sequence
-
Breast Neoplasms / genetics
-
DNA Primers
-
Female
-
Humans
-
In Situ Hybridization, Fluorescence
-
Molecular Sequence Data
-
Neoplasm Proteins / genetics*
-
Neoplasm Proteins / metabolism*
-
Nuclear Proteins*
-
Polymerase Chain Reaction
-
Polymorphism, Genetic*
-
Proto-Oncogene Proteins c-mdm2
-
Proto-Oncogene Proteins*
-
Saccharomyces cerevisiae / genetics
Substances
-
DNA Primers
-
Neoplasm Proteins
-
Nuclear Proteins
-
Proto-Oncogene Proteins
-
MDM2 protein, human
-
Proto-Oncogene Proteins c-mdm2