Abstract
Human immunodeficiency virus isolates express a Nef protein with either an alanine or a threonine at amino acid residue 15. The threonine residue is a site for phosphorylation by protein kinase C. Jurkat T cells constitutively expressing the alanine variant of Nef exhibit the ability to downregulate the induction of transcription factors NF-kB and AP-1. In contrast, Jurkat cells with the threonine variant of Nef are at least partially restored in their ability to recruit NF-kB and AP-1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Binding Sites
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DNA, Viral / metabolism
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Down-Regulation
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Electrophoresis, Polyacrylamide Gel / methods
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Gene Products, nef / analysis
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Gene Products, nef / chemistry*
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Gene Products, nef / physiology*
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Genes, nef / genetics
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HIV / chemistry
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HIV / metabolism*
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HIV Long Terminal Repeat / genetics
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Humans
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Interleukin-2 / genetics
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NF-kappa B / biosynthesis*
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Phosphorylation
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Point Mutation
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Promoter Regions, Genetic / genetics
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Proto-Oncogene Proteins c-jun / biosynthesis*
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Threonine / metabolism
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Transcription, Genetic
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Tumor Cells, Cultured
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nef Gene Products, Human Immunodeficiency Virus
Substances
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DNA, Viral
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Gene Products, nef
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Interleukin-2
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NF-kappa B
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Proto-Oncogene Proteins c-jun
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nef Gene Products, Human Immunodeficiency Virus
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Threonine