Maintenance of the pluripotential phenotype of embryonic stem cells through direct activation of gp130 signalling pathways

K Yoshida; I Chambers; J Nichols; A Smith; M Saito; K Yasukawa; M Shoyab; T Taga; T Kishimoto

doi:10.1016/0925-4773(94)90030-2

Maintenance of the pluripotential phenotype of embryonic stem cells through direct activation of gp130 signalling pathways

Mech Dev. 1994 Feb;45(2):163-71. doi: 10.1016/0925-4773(94)90030-2.

Authors

K Yoshida¹, I Chambers, J Nichols, A Smith, M Saito, K Yasukawa, M Shoyab, T Taga, T Kishimoto

Affiliation

¹ Institute for Molecular and Cellular Biology, Osaka University, Japan.

PMID: 8199053
DOI: 10.1016/0925-4773(94)90030-2

Abstract

Propagation of the undifferentiated pluripotential phenotype of embryonic stem (ES) cells is dependent on the cytokine differentiation inhibiting activity/leukemia inhibitory factor (DIA/LIF). The DIA/LIF receptor complex is a heterodimer of DIA/LIF receptor (DIA/LIF-R) and gp130. The latter is also a component of the interleukin-6 (IL-6) receptor complex. We report that a combination of IL-6 and soluble IL-6 receptor (sIL-6R), which can induce homodimerisation of gp130 and activation of signalling processes, sustains self-renewal of pluripotential ES cells. Our findings indicate that the IL-6/sIL-6R complex acts on ES cells through gp130 alone, bypassing DIA/LIF-R, and therefore implicate gp130 as the key component in the signalling pathway responsible for stem cell renewal.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antigens, CD*
Blastocyst / cytology
Cell Differentiation
Cells, Cultured
Chimera
Cytokine Receptor gp130
Female
Gene Expression Regulation
Growth Inhibitors / pharmacology
Growth Inhibitors / physiology*
Interleukin-6*
Leukemia Inhibitory Factor
Leukemia Inhibitory Factor Receptor alpha Subunit
Lymphokines / pharmacology
Lymphokines / physiology*
Male
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / physiology*
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Phenotype
Receptors, Cytokine / chemistry
Receptors, Cytokine / physiology*
Receptors, Interleukin / chemistry
Receptors, Interleukin / physiology*
Receptors, Interleukin-6
Receptors, OSM-LIF
Signal Transduction*
Stem Cells / cytology*
Stem Cells / drug effects
Stem Cells / physiology

Substances

Antigens, CD
Growth Inhibitors
Il6st protein, mouse
Interleukin-6
Leukemia Inhibitory Factor
Leukemia Inhibitory Factor Receptor alpha Subunit
Lif protein, mouse
Lifr protein, mouse
Lymphokines
Membrane Glycoproteins
Receptors, Cytokine
Receptors, Interleukin
Receptors, Interleukin-6
Receptors, OSM-LIF
Cytokine Receptor gp130