Abstract
We have studied the expression and regulation of the interleukin-6 receptor (gp80) and its signal transducer gp130 in primary human blood monocytes. Here, we show that freshly isolated human monocytes express mRNAs for gp80 and gp130. In contrast to a previous report [(1989) FEBS Lett. 249, 27-30] we find that neither lipopolysaccharide nor interleukin-6 (IL-6) lead to a down-regulation of IL-6 receptor mRNA in monocytes. Also in the human monocytic cell line Mono Mac 6 no effect of IL-6 on receptor mRNA levels was observed. For signal transducer gp130 mRNA in monocytes a small and transient up-regulation by IL-6 was found.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Blotting, Northern
-
Cell Line
-
Cells, Cultured
-
Down-Regulation
-
Gene Expression Regulation / drug effects*
-
Humans
-
Interleukin-6 / biosynthesis*
-
Interleukin-6 / pharmacology*
-
Leukemia, Monocytic, Acute
-
Lipopolysaccharides / pharmacology
-
Monocytes / drug effects
-
Monocytes / immunology
-
Monocytes / metabolism*
-
RNA, Messenger / biosynthesis*
-
Receptors, Interleukin / biosynthesis*
-
Receptors, Interleukin-6
-
Recombinant Proteins / pharmacology
-
Signal Transduction
-
Transcription, Genetic / drug effects
-
Transforming Growth Factor beta / pharmacology
-
Tumor Cells, Cultured
Substances
-
Interleukin-6
-
Lipopolysaccharides
-
RNA, Messenger
-
Receptors, Interleukin
-
Receptors, Interleukin-6
-
Recombinant Proteins
-
Transforming Growth Factor beta