Improved method of preparation of inflated-fixed lung for radiologic-pathologic correlation

J Thorac Imaging. 1994 Spring;9(2):112-5. doi: 10.1097/00005382-199421000-00012.

Abstract

The technique of inflation and fixation of the lung with polyethylene glycol is useful for specimen radiography and radiologic-pathologic correlation, but it is limited by poor histologic staining of the fixed tissue. To improve staining we used formalin to distend and fix the lung before standard fixation with a polyethylene glycol mixture. In this preliminary study, canine and infant lungs, and lungs from three cases of lung cancer were examined. The modified technique provided high-quality staining and satisfactory specimen radiography in all cases except one of the lung cancers; in this case excessive shrinkage occurred and degraded radiographic quality. We conclude that the new method of preparation permits both specimen radiography and high quality staining of the fixed tissue.

MeSH terms

  • Adult
  • Animals
  • Dogs
  • Formaldehyde
  • Humans
  • Immunohistochemistry
  • Infant
  • Lung / diagnostic imaging*
  • Lung / pathology*
  • Lung Neoplasms / diagnostic imaging
  • Lung Neoplasms / pathology
  • Polyethylene Glycols
  • Tissue Fixation*
  • Tomography, X-Ray Computed

Substances

  • Formaldehyde
  • Polyethylene Glycols