The smallest known eukaryotic genomes encode a protein gene: towards an understanding of nucleomorph functions

Mol Gen Genet. 1994 Jun 3;243(5):600-4. doi: 10.1007/BF00284209.

Abstract

Cryptomonads are unicellular algae with plastids surrounded by four membranes. Between the two pairs of membranes lies a periplastidal compartment that harbours a DNA-containing organelle, termed the nucleomorph. The nucleomorph is the vestigial nucleus of a phototrophic, eukaryotic endosymbiont. Subcloning of parts of one nucleomorph chromosome revealed a gene coding for an Hsp70 protein. We demonstrate the expression of this nucleomorph protein-coding gene and present a model for protein transport from the host to the endosymbiont compartment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Cell Membrane
  • Electrophoresis, Gel, Pulsed-Field
  • Eukaryota / genetics*
  • Eukaryotic Cells
  • Genes, Plant*
  • Genomic Library
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics*
  • Organelles*
  • Phylogeny
  • Plant Proteins / genetics*
  • Plastids
  • Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Symbiosis / physiology*

Substances

  • Heat-Shock Proteins
  • Plant Proteins
  • Proteins

Associated data

  • GENBANK/X72621