Abstract
The S protein of hemagglutinating encephalomyelitis virus is shown to be a hemagglutinin requiring N-acetyl-9-O-acetylneuraminic acid as a receptor determinant on the surface of erythrocytes. The ability of bovine coronavirus to recognize 9-O-acetylated sialic acid was used to establish a binding assay for the detection of glycoproteins containing this type of sugar. The assay is very fast, because it uses the acetylesterase of the viral HE protein to localize bound virus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biological Assay
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Blood Proteins / analysis
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Cattle
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Cell Line
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Chickens
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Coronavirus / metabolism*
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Coronavirus, Bovine / metabolism*
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Dogs
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Glycoproteins / analysis
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Hemagglutination Tests
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Hemagglutinins, Viral / metabolism*
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Membrane Glycoproteins / metabolism*
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Protein Binding
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Rats
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Receptors, Coronavirus
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Receptors, Virus / metabolism*
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Sialic Acids / analysis
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Sialic Acids / metabolism*
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Spike Glycoprotein, Coronavirus
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Viral Envelope Proteins / metabolism*
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Viral Fusion Proteins*
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Viral Proteins / metabolism
Substances
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Blood Proteins
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Glycoproteins
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Hemagglutinins, Viral
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Membrane Glycoproteins
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Receptors, Coronavirus
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Receptors, Virus
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Sialic Acids
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Spike Glycoprotein, Coronavirus
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Viral Envelope Proteins
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Viral Fusion Proteins
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Viral Proteins
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hemagglutinin esterase
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spike glycoprotein, SARS-CoV
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spike protein, mouse hepatitis virus
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9-O-acetyl-N-acetylneuraminic acid