Abstract
Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3-SLA, respectively), which largely overcome this problem and yield readily interpretable banding patterns.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenine
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Alleles
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Base Sequence
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Cytosine
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DNA / analysis*
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DNA Primers
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Electrophoresis, Polyacrylamide Gel / methods
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Female
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Genetic Markers
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Genotype
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Humans
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Male
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Molecular Sequence Data
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Pedigree
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Polymerase Chain Reaction / methods*
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Polymorphism, Genetic*
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Repetitive Sequences, Nucleic Acid*
Substances
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DNA Primers
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Genetic Markers
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Cytosine
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DNA
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Adenine