Abstract
We have previously characterized an infectious mouse mammary tumor virus [(MMTV(SW)] which induces a strong superantigen response in vivo. Here we describe the isolation and characterization of MMTV(C4) which was derived from milk of mice implanted with hyperplastic alveolar nodules. MMTV(C4) stimulates V beta 2 expressing T cells after local injection in vivo. Comparison with known open reading frame (orf) sequences revealed high homology to Mtv-6, an endogenous virus interacting with V beta 3-expressing T cells. The carboxyl-terminal amino acids were, however, altered. High homology including the carboxyl-terminal orf amino acids were found with MMTV(C3H-K). We show here that MMTV(C3H-K) has lost its superantigen function. Sequence comparisons permitted the characterization of few key amino acids which could be important for T cell receptor interaction and superantigen processing.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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DNA, Viral / genetics
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Female
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Hyperplasia
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Mammary Glands, Animal / immunology
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Mammary Glands, Animal / microbiology*
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Mammary Glands, Animal / pathology
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Mammary Neoplasms, Experimental / genetics
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Mammary Neoplasms, Experimental / immunology
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Mammary Neoplasms, Experimental / microbiology
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Mammary Tumor Virus, Mouse / genetics
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Mammary Tumor Virus, Mouse / immunology
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Mammary Tumor Virus, Mouse / isolation & purification*
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Mice
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Mice, Inbred BALB C
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Milk / microbiology*
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Molecular Sequence Data
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Neoplasm Transplantation
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Precancerous Conditions / genetics
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Precancerous Conditions / immunology
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Precancerous Conditions / microbiology
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Sequence Deletion
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Viral Proteins / genetics
Substances
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DNA, Viral
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Receptors, Antigen, T-Cell, alpha-beta
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Viral Proteins
Associated data
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GENBANK/Z22552
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GENBANK/Z25442
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GENBANK/Z25443
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GENBANK/Z25444
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GENBANK/Z25445
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GENBANK/Z25446
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GENBANK/Z25447
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GENBANK/Z25448
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GENBANK/Z25449
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GENBANK/Z25450