The V kappa genes of the L regions and the repertoire of V kappa gene sequences in the human germ line

Eur J Immunol. 1993 Nov;23(11):2868-75. doi: 10.1002/eji.1830231121.

Abstract

Only 14 of the 25 V kappa genes and pseudogenes had been found before as parts of the L regions. The cloning and linking described in the accompanying report allowed us now to assign to Lp or Ld some V kappa genes which had been found before on scattered clones. In addition the sequences of several still unknown genes are reported here, thus completing the publication of the V kappa genes of the kappa locus as far as they are potentially functional or have only one or two 1-bp defects. Of the V kappa genes of the kappa locus, 32 are potentially functional, 16 have minor defects, 3 have both potentially functional and slightly defective alleles and 25 are pseudogenes which amounts to a repertoire of 76 V kappa-related gene sequences. The V kappa genes of the L regions are, within the subgroups, particularly similar to each other, which is in part due to common evolutionary origins and in part caused by gene conversion-like events. One donor-acceptor pair could be clearly identified, since converted and not-converted alleles of the acceptor gene were found. In other cases the duplicates of the converted genes served as non-converted controls.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Antibody Diversity
  • Base Sequence
  • Biological Evolution
  • DNA / genetics
  • Gene Conversion
  • Genes, Immunoglobulin*
  • Humans
  • Immunoglobulin Variable Region / genetics*
  • Immunoglobulin kappa-Chains / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Restriction Mapping

Substances

  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains
  • DNA

Associated data

  • GENBANK/V00558
  • GENBANK/V01577
  • GENBANK/X01668
  • GENBANK/X02725
  • GENBANK/X17262
  • GENBANK/X17263
  • GENBANK/X17264
  • GENBANK/X17265
  • GENBANK/X63392
  • GENBANK/X72808
  • GENBANK/X72810
  • GENBANK/X72811
  • GENBANK/X72812
  • GENBANK/X72813
  • GENBANK/X72814
  • GENBANK/X72815
  • GENBANK/X72816
  • GENBANK/X72817
  • GENBANK/X72818
  • GENBANK/X72819
  • GENBANK/X72820