Does phosphoglucoisomerase display anomeric specificity or selectivity towards alpha-D-glucose 6-phosphate? An assessment by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r

F Kayser; M Biesemans; R Willem; F Malaisse-Lagae; W J Malaisse

doi:10.1042/bj2950607

Does phosphoglucoisomerase display anomeric specificity or selectivity towards alpha-D-glucose 6-phosphate? An assessment by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r

Biochem J. 1993 Oct 15;295 ( Pt 2)(Pt 2):607-9. doi: 10.1042/bj2950607.

Authors

F Kayser¹, M Biesemans, R Willem, F Malaisse-Lagae, W J Malaisse

Affiliation

¹ High Resolution NMR Centre, Faculty of Engineering, Free University of Brussels (VUB), Belgium.

Abstract

The study by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r. of hexose 6-phosphates interconversion in the reaction catalysed by yeast phosphoglucoisomerase reveals that the enzyme displays anomeric selectivity, rather than specificity, towards alpha-D-glucose 6-phosphate. Indeed, beta-D-glucose 6-phosphate participates for about 20% to the total and direct conversion of the aldohexose into oxohexose ester.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Glucose-6-Phosphate
Glucose-6-Phosphate Isomerase / chemistry
Glucose-6-Phosphate Isomerase / metabolism*
Glucosephosphates / chemistry
Glucosephosphates / metabolism*
Kinetics
Magnetic Resonance Spectroscopy / methods
Molecular Conformation
Phosphorus Isotopes
Substrate Specificity

Substances

Glucosephosphates
Phosphorus Isotopes
Glucose-6-Phosphate
Glucose-6-Phosphate Isomerase