Isolation of an immunosuppressive lectin from Phaseolus vulgaris L. cv Cacahuate using stroma

Prep Biochem. 1993 Nov;23(4):473-83. doi: 10.1080/10826069308544570.

Abstract

An immunosuppressive lectin was isolated from seed of Phaseolus vulgaris cv Cacahuate using physically entrapped stroma. The lectin was found to be a 94 kDa tetrameric protein. When 50 micrograms, of this lectin were administered intraperitoneally 2 days before the immunization with sheep red blood cells, humoral response against the immunogen was completely inhibited. Other properties of the protein are discussed.

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Carbohydrates / analysis
  • Cattle
  • Edetic Acid / pharmacology
  • Erythrocytes / immunology
  • Fabaceae*
  • Goats
  • Guinea Pigs
  • Hemagglutination
  • Humans
  • Immunosuppression Therapy
  • Immunosuppressive Agents*
  • Macromolecular Substances
  • Mice
  • Molecular Weight
  • Perissodactyla
  • Phytohemagglutinins / chemistry
  • Phytohemagglutinins / isolation & purification*
  • Phytohemagglutinins / pharmacology
  • Plant Lectins
  • Plants, Medicinal*
  • Rabbits
  • Rats
  • Seeds / chemistry*
  • Sheep
  • Temperature

Substances

  • Amino Acids
  • Carbohydrates
  • Immunosuppressive Agents
  • Macromolecular Substances
  • Phytohemagglutinins
  • Plant Lectins
  • Edetic Acid