Abstract
Characterization of Brn-3.0 and identification of a highly related member (Brn-3.1) of the class IV POU-domain family suggest potential roles of Brn-3.0 in the development of retinal ganglion cells and sensory neurons, as well as potential roles in the pituitary gland and the immune system. Brn-3.0 is expressed in the pituitary gland and in a corticotroph cell line. A functional DNA response element has been identified in the proopiomelanocortin promoter. In contrast to previously described mammalian POU-domain proteins, Brn-3.0 binds relatively ineffectively to known octamer DNA motifs, but instead binds with high affinity to a distinct set of DNA elements, functioning as a transcriptional activator. Brn-3.0, Brn-3.1, and the Drosophila tI-POU share an N-terminal region of homology, referred to as the "POU-IV box," which is similar to a conserved functional domain in the c-myc gene family.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Age Factors
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Animals
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Base Sequence
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Cloning, Molecular
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DNA Mutational Analysis
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DNA Primers / chemistry
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DNA, Complementary / genetics
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DNA-Binding Proteins / genetics*
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Endocrine Glands / embryology
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Endocrine Glands / metabolism
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Gene Expression
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Genes
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Lymphoid Tissue / embryology
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Lymphoid Tissue / metabolism
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Mice / embryology
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Molecular Sequence Data
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Multigene Family
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Nerve Tissue Proteins / genetics
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RNA, Messenger / genetics
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Regulatory Sequences, Nucleic Acid*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Structure-Activity Relationship
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Transcription Factor Brn-3
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Transcription Factor Brn-3A
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Transcription Factors / genetics*
Substances
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DNA Primers
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DNA, Complementary
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DNA-Binding Proteins
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Nerve Tissue Proteins
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POU4F1 protein, human
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Pou4f1 protein, mouse
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RNA, Messenger
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Transcription Factor Brn-3
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Transcription Factor Brn-3A
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Transcription Factors