Enhancement of antineoplastic effect and attenuation of sister chromatid exchanges by prostaglandin E2 in Ehrlich ascites tumour cells treated with cyclophosphamide in vivo

Prostaglandins Leukot Essent Fatty Acids. 1993 Sep;49(3):707-10. doi: 10.1016/0952-3278(93)90082-8.

Abstract

Reduced sister chromatid exchanges (SCE) frequency in response to cyclophosphamide (CP) was observed when Ehrlich ascites tumour (EAT) cells were exposed in vivo to 2 micrograms/g body weight of prostaglandin E2 (PGE2). 1 h before i.p. injection of 5-bromodeoxyuridine (BrdUrd) adsorbed to activated charcoal, EAT-bearing mice treated i.p. with CP appeared to have increased SCE rates and cell division delays. PGE2 had no effect on survival and in inhibiting tumour growth. CP had only a slight non-significant effect on survival and in inhibiting tumour growth. In mice treated with the combined CP (5 micrograms/g bd wt) plus PGE2 (2 micrograms/g bd wt) a significant enhancement (P < 0.01) of survival time was accompanied by inhibition of tumour growth (P < 0.01) in comparison with the untreated controls. These data imply that SCEs might result from errors in a repair process which might involve a PGE2 sensitive step.

MeSH terms

  • Animals
  • Bromodeoxyuridine
  • Carcinoma, Ehrlich Tumor / drug therapy*
  • Carcinoma, Ehrlich Tumor / genetics
  • Cyclophosphamide / pharmacology*
  • Dinoprostone / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Sister Chromatid Exchange / drug effects*
  • Tumor Cells, Cultured

Substances

  • Cyclophosphamide
  • Bromodeoxyuridine
  • Dinoprostone