This review article is designed to expose physical therapists to an examination of muscle organization and the implications that this organization has for therapeutic applications. The partitioning hypothesis is based on the fact that an individual muscle is arranged in a more complex array than simply fibers attaching at aponeuroses, tendons, or bones with a single muscle nerve innervation. Neuromuscular compartments, which are distinct subvolumes of a muscle, each innervated by an individual muscle nerve branch and each containing motor unit territories with a unique array of physiological attributes, are described. In addition, the organization of individual muscles into these subunits is paralleled by the organization of their parent motoneurons within the spinal cord. These notions are detailed in a review of data derived from studies performed primarily in cat and rat models. Recent data derived from morphological and anatomical study of human muscles support the existence of similar neuromuscular partitions. These data are complemented by physiological studies, the results from which suggest that partitions may have functional or task-oriented roles; that is, different portions of one muscle may be called into play depending on the task demands of the situation. The importance of these observations for reconsidering how we provide clinical applications, such as neuromuscular stimulation or kinesiological monitoring, is discussed.