Abstract
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a recently identified potent vascular smooth muscle cell (SMC) mitogen. We investigated the effect of shear stress on human HB-EGF mRNA levels in cultured human umbilical vein endothelial cells (HUVEC). In response to shear stress (8 dyne/cm2), HB-EGF mRNA levels in HUVEC increased rapidly, peaked at 3 h, and returned to near base line at 7 h. The shear stress-induced HB-EGF gene expression in HUVEC is completely blocked by 12-O-tetra-decanoylphorbol-13-acetate pre-treatment, suggesting the induction of HB-EGF is mediated by protein kinase C.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Northern
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Calcium / metabolism
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Cells, Cultured
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DNA Probes
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Egtazic Acid / analogs & derivatives
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Egtazic Acid / pharmacology
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Endothelium, Vascular / cytology
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Endothelium, Vascular / metabolism*
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Epidermal Growth Factor / genetics*
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Gene Expression Regulation*
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Heparin-binding EGF-like Growth Factor
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Humans
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Intercellular Signaling Peptides and Proteins
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Physical Stimulation
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Protein Kinase C / metabolism
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RNA, Messenger / biosynthesis*
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Signal Transduction*
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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DNA Probes
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HBEGF protein, human
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Heparin-binding EGF-like Growth Factor
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Intercellular Signaling Peptides and Proteins
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RNA, Messenger
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1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester
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Egtazic Acid
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Epidermal Growth Factor
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Protein Kinase C
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Tetradecanoylphorbol Acetate
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Calcium