Soft agar cloning with antibody overlay to identify Chinese hamster ovary clones secreting recombinant products

Biotechniques. 1993 Oct;15(4):594, 597.
No abstract available

MeSH terms

  • Animals
  • CHO Cells / metabolism*
  • Cloning, Molecular / methods*
  • Cricetinae
  • Enzyme-Linked Immunosorbent Assay
  • Immunoglobulins / analysis
  • Immunoglobulins / genetics
  • Recombinant Proteins / metabolism*
  • Sepharose
  • Transfection

Substances

  • Immunoglobulins
  • Recombinant Proteins
  • Sepharose