Abstract
The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-ribosylation factor (ARF), helps to provide vectoriality to vesicle transport. Coat assembly is triggered when ARF binds GTP, initiating transport vesicle budding, and coat disassembly is triggered when ARF hydrolyzes GTP, allowing the uncoated vesicle to fuse.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ADP-Ribosylation Factors
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Animals
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CHO Cells
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Carrier Proteins / metabolism*
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Cell-Free System
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Cricetinae
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GTP-Binding Proteins / metabolism*
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Guanosine Triphosphate / metabolism*
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Membrane Fusion
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Microscopy, Electron
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Mutagenesis, Site-Directed
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Recombinant Proteins
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Structure-Activity Relationship
Substances
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Carrier Proteins
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Recombinant Proteins
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Guanosine Triphosphate
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GTP-Binding Proteins
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ADP-Ribosylation Factors