Abstract
To facilitate the screening for clones of transfected packaging cells producing a high yield of recombinant retrovirus, we present a fast and simple method for the isolation of overexpressing cells. By this method the efficiency of virus production can generally be enhanced 10- to 100-fold by application of high selection pressure. Cell lines which exhibit titers of up to 10(8) CFU/ml were obtained.
MeSH terms
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3T3 Cells
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Animals
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Cell Line
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Cloning, Molecular / methods*
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Drug Resistance, Microbial / genetics
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Genetic Markers
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Leukemia Virus, Murine / genetics
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Mice
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Neomycin / pharmacology
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Proviruses / genetics
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Puromycin / pharmacology
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Retroviridae / genetics*
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Retroviridae / growth & development*
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Selection, Genetic
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Tetrahydrofolate Dehydrogenase / genetics
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Virus Cultivation
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Virus Replication
Substances
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Genetic Markers
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Puromycin
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Tetrahydrofolate Dehydrogenase
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Neomycin