Abstract
Coding and noncoding sequences were analyzed from field and experimental isolates of Borna disease virus. For a 24-kDa protein, maximum divergence was 1.5% at the predicted amino acid level and 3.1% at the nucleotide level. For a 40-kDa protein, maximum divergence was 1.1% at the predicted amino acid level and 4.1% at the nucleotide level. The highest variability in sequence (10%) was found in a 40-nucleotide stretch of genomic RNA between coding sequences for the 40- and 24-kDa proteins. The degree of sequence conservation in these isolates, passaged in various host species in vivo and in vitro over a period of 64 years, is unusual for negative-strand RNA viruses.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Borna Disease / microbiology
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Borna disease virus / genetics*
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Brain / microbiology
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Cloning, Molecular
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Conserved Sequence
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Genetic Variation
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Germany
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Horse Diseases / microbiology
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Horses / microbiology
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Molecular Sequence Data
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RNA, Viral / genetics*
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Serial Passage
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Viral Proteins / chemistry
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Viral Proteins / genetics*
Substances
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RNA, Viral
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Viral Proteins
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p24 protein, Borna disease virus
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p40 protein, Borna disease virus
Associated data
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GENBANK/S67502
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GENBANK/S67507
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GENBANK/S67513