Abstract
Using an upright microscope and an interface type slice chamber for epifluorometric imaging of fura-2 injected neurons in brain slice is particularly convenient to obtain good spatial resolution. Using this approach we found that muscarinic activation uncouples K-channel activation from intradendritic Ca and that postsynaptic spines represent independent compartments for Ca-activated processes when activated synaptically.
MeSH terms
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Animals
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Calcium / physiology*
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Dendrites / physiology*
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Fluorometry
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Fura-2
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Guinea Pigs
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Hippocampus / cytology*
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Hippocampus / metabolism
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Hippocampus / physiology
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In Vitro Techniques
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Neurons / physiology*
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Potassium Channels / physiology
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Pyramidal Cells / metabolism
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Receptors, Muscarinic / drug effects
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Receptors, Muscarinic / metabolism
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Signal Transduction / physiology*
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Synapses / metabolism
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Synapses / physiology
Substances
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Potassium Channels
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Receptors, Muscarinic
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Calcium
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Fura-2