The carrot secreted glycoprotein gene EP1 is expressed in the epidermis and has sequence homology to Brassica S-locus glycoproteins

Plant J. 1993 Nov;4(5):855-62. doi: 10.1046/j.1365-313x.1993.04050855.x.

Abstract

Non-embryogenic carrot suspension cells secrete the EP1 glycoprotein. A cDNA clone encoding EP1 was isolated and sequenced. The EP1 sequence revealed a region of homology with Brassica S-locus glycoprotein genes, an Arabidopsis S-like gene and putative S-like receptor protein kinases from maize and Arabidopsis. EP1 gene expression, analysed by in situ mRNA localization, was detected in cells located at the surface of the seedling: in the epidermis of the root, the hypocotyl and the cotyledons, in the root cap, and in a crescent of cells in the apical dome of the shoot. In developing seeds, expression was most pronounced in both the inner and outer integument epidermis.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Complementary / genetics
  • Genes, Plant*
  • Glycoproteins / genetics*
  • Glycoproteins / metabolism
  • In Situ Hybridization
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • RNA, Messenger / isolation & purification
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Vegetables / genetics*

Substances

  • DNA, Complementary
  • EP1 protein, Daucus carota
  • Glycoproteins
  • Plant Proteins
  • RNA, Messenger
  • self-incompatibility locus specific glycoprotein, Brassica

Associated data

  • GENBANK/L16983
  • GENBANK/X72085