50 patients undergoing biliary surgery were studied to know the incidence of fungal infection. Samples such as bile & stones for mycological analysis were obtained from the gall bladder & Common Bile Duct (CBD) at the time of surgery. Fungal aetiology was established in 8 (16.3%) cases. Incidence of fungal infection was more (12.2%) in gall bladder bile as compared to 6% in common bile duct bile. The nucleus of stone yielded growth of fungus in only 2 (4.6%) cases. Candida albicans, C. tropicalies, C, kruzei, Penicillium and Aspergillus were the common fungal isolates in the present study.