Abstract
cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism*
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CHO Cells
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Carrier Proteins / biosynthesis*
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Cattle
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Chromatography, Gel
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Clathrin / biosynthesis*
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Cricetinae
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Cytosol / metabolism
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DNA Primers
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DNA, Complementary / metabolism
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GTP-Binding Proteins / biosynthesis
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GTP-Binding Proteins / metabolism*
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Golgi Apparatus / drug effects
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
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Intracellular Membranes / metabolism*
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Intracellular Membranes / ultrastructure
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Liver / metabolism*
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Macromolecular Substances
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Membrane Proteins / biosynthesis
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Molecular Weight
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Oligonucleotides, Antisense
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Polymerase Chain Reaction
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Restriction Mapping
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rhoB GTP-Binding Protein
Substances
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Carrier Proteins
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Clathrin
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DNA Primers
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DNA, Complementary
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Macromolecular Substances
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Membrane Proteins
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Oligonucleotides, Antisense
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Guanosine 5'-O-(3-Thiotriphosphate)
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GTP-Binding Proteins
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rhoB GTP-Binding Protein