Non-peptide angiotensin II receptor antagonists. 1. Design, synthesis, and biological activity of N-substituted indoles and dihydroindoles

J Med Chem. 1993 Dec 24;36(26):4230-8. doi: 10.1021/jm00078a013.

Abstract

A series of N-acylated indoles (12-18), N-alkylated indoles (19-24), N-acylated dihydroindoles (26-30), and N-alkylated dihydroindoles (31-34) were synthesized and evaluated in the in vitro AT1 (rabbit aorta) and AT2 (rat midbrain) binding assay. The carboxylic acid 3-[[N-(2-carboxy-3,6-dichlorobenzoyl)-5-indolyl]methyl]-5,7-dimeth yl- 2-ethyl-3H-imidazo[4,5-b]pyridine (14b) was found to be the most potent AT1 (IC50 = 0.8 nM) antagonist in the N-acylated indole series and displayed a 25-fold higher potency than the parent unsubstituted derivative 14a (AT1 IC50 = 20 nM) and a 22-fold greater potency than the corresponding dihydroindole analog 27 (AT1 IC50 = 18 nM). Replacement of the terminal carboxyl (COOH) of 14a with the bioisostere tetrazole in 16 (AT1 IC50 = 5 nM, AT2 IC50 = 130 nM) not only improved the AT1 potency by 4-fold but also resulted in a 50-fold increase in AT2 activity. In the N-alkylated indole series, the tetrazole 3-[[N-(2-tetrazol-5-yl-6-chlorobenzyl)-5- indolyl]methyl]-5,7-dimethyl-2-ethyl-3H-imidazo[4,5-b]pyridine (24) exhibited the highest AT1 (IC50 = 1 nM) activity, revealing a 230-fold increase in AT1 activity as a result of the incorporation of the isosteric tetrazole for the carboxyl (COOH) of 20 and a nearly 9-fold increase over the corresponding deschloro analog 22 (AT1 IC50 = 8.7 nM). Tetrazole 34 was identified as the most potent (AT1 IC50 = 18 nM) AT1 receptor antagonist in a structurally distinct series of compounds derived from N-alkylation of dihydroindole 25. A new class of highly potent (14b, AT1 IC50 = 0.8 nM; 24, AT1 IC50 = 1 nM) AT1-selective non-peptide AII receptor antagonists derived from N-substituted indoles and dihydroindoles is disclosed. Tetrazole 24 of the N-alkylated indole series displayed good in vivo activity by blocking the AII-induced pressor response for 5.5 h after intravenous administration in conscious normotensive rats at a 1.0 mg/kg dose level.

Publication types

  • Comparative Study

MeSH terms

  • 1-Sarcosine-8-Isoleucine Angiotensin II / metabolism
  • Acylation
  • Alkylation
  • Angiotensin II / pharmacology
  • Angiotensin Receptor Antagonists*
  • Animals
  • Aorta / metabolism
  • Benzoates
  • Blood Pressure / drug effects
  • Drug Design
  • Imidazoles / chemical synthesis*
  • Imidazoles / metabolism
  • Imidazoles / pharmacology
  • Indoles / chemical synthesis*
  • Indoles / metabolism
  • Indoles / pharmacology
  • Kinetics
  • Mesencephalon / metabolism
  • Molecular Structure
  • Pyridines / chemical synthesis*
  • Pyridines / metabolism
  • Pyridines / pharmacology
  • Rabbits
  • Rats
  • Receptors, Angiotensin / metabolism
  • Structure-Activity Relationship

Substances

  • Angiotensin Receptor Antagonists
  • Benzoates
  • Imidazoles
  • Indoles
  • Pyridines
  • Receptors, Angiotensin
  • Angiotensin II
  • 3-((N-(2-tetrazol-5-yl-6-chlorobenzyl)-5-indolyl)methyl)-5,7-dimethyl-2-ethyl-3H-imidazo(4,5-b)pyridine
  • 1-Sarcosine-8-Isoleucine Angiotensin II