A case of trisomy 22 in a newborn calf is described. Previously, this anomaly has only been identified in one calf. The calf had several defects including brachygnathia superior, malformation of septum nasi and conchae, and an interventricular septal defect. Chromosome analysis was performed on cultured blood lymphocytes, and in all counted metaphases 61 chromosomes were present. The extra chromosome was identified as a chromosome 22 by R-banding.