Crystallization and preliminary X-ray diffraction analysis of the beta-chain of a T-cell antigen receptor

J Mol Biol. 1994 Jan 14;235(2):795-7. doi: 10.1006/jmbi.1994.1037.

Abstract

A secreted form of the beta-chain of a T-cell receptor specific for a hemagglutinin peptide of influenza virus in the context of the major histocompatibility complex class II I-Ed molecule has been crystallized in a form suitable for X-ray diffraction analysis. The crystals are tetragonal, space group P4(1)2(1)2 (or P4(3)2(1)2), with cell dimensions a = b = 71.4 A, c = 312.9 A, and diffract to beyond 3.5 A resolution. The beta-chain appears to behave as a stable homodimer in solution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Crystallization
  • Crystallography, X-Ray
  • Molecular Sequence Data
  • Receptors, Antigen, T-Cell / chemistry*

Substances

  • Receptors, Antigen, T-Cell