Multidose use of exametazine for leukocyte labelling: a new approach using tin enhancement

Nucl Med Commun. 1993 Nov;14(11):1035-40. doi: 10.1097/00006231-199311000-00016.

Abstract

Reconstituted exametazime is inherently unstable due to the rapid oxidation of the small amount (7.6 micrograms) of stannous chloride present in the kit. A novel approach has been used to improve the shelf-life of reconstituted exametazime based on the premise that tin lost through oxidation should be replaced by further amounts of tin. Freshly prepared stannous solution, 0.1 ml (0.66 microgram Sn2+), was mixed with 0.3 ml (25 micrograms) exametazime solution followed by 400-500 MBq pertechnetate. Mixed leukocytes from 50 ml of patients' blood were labelled with 99Tcm-exametazime which had been prepared by the tin enhancement. One hundred and fourteen patients were investigated. The median labelling efficiency was 72%, range 30-96%. The exametazime had been reconstituted up to 5 months previously. Because of the small volumes of the exametazime solution used, up to 15 doses for leukocyte labelling could be prepared from one vial.

MeSH terms

  • Butanones*
  • Colitis, Ulcerative / diagnostic imaging
  • Crohn Disease / diagnostic imaging
  • Humans
  • Isotope Labeling / methods*
  • Leukocytes*
  • Radionuclide Imaging
  • Technetium*

Substances

  • Butanones
  • Technetium