Abstract
The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Bacterial Proteins
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Cloning, Molecular
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Genes, Bacterial
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Hemolysis
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In Vitro Techniques
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Mutagenesis
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Point Mutation
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Sheep
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Streptococcus pneumoniae / genetics
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Streptococcus pneumoniae / pathogenicity
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Streptolysins / genetics*
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Streptolysins / toxicity*
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Virulence / genetics
Substances
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Bacterial Proteins
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Streptolysins
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plY protein, Streptococcus pneumoniae