HPLC determination of inorganic cation levels in CSF and plasma of conscious rabbits

J Pharmacol Toxicol Methods. 1993 Apr;29(2):99-104. doi: 10.1016/1056-8719(93)90057-l.

Abstract

An HPLC method is described using conductimetric detection for the quantitative determination of sodium, potassium, magnesium, and calcium in cerebrospinal fluid (CSF) and plasma of conscious rabbits. This method enabled the four cations to be estimated with rapidity, sensitivity, accuracy, and precision. The mean millimolar concentrations +/- SD found in CSF and (plasma) of 15 untreated animals were as follows: sodium, 146.96 +/- 17.84 (135.06 +/- 20.11); potassium, 3.32 +/- 0.56 (4.57 +/- 1.03); magnesium, 0.90 +/- 0.20 (0.72 +/- 0.13); and calcium, 1.47 +/- 0.19 (3.32 +/- 0.59).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / blood
  • Calcium / cerebrospinal fluid
  • Cations / blood*
  • Cations / cerebrospinal fluid*
  • Chromatography, High Pressure Liquid*
  • Magnesium / blood
  • Magnesium / cerebrospinal fluid
  • Male
  • Potassium / blood
  • Potassium / cerebrospinal fluid
  • Rabbits
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sodium / blood
  • Sodium / cerebrospinal fluid

Substances

  • Cations
  • Sodium
  • Magnesium
  • Potassium
  • Calcium