Abstract
The production of the leukemic cell-growth-promoting factor (LGF) in TGF-beta 1-treated fibroblast cells was studied. BALB/c3T3 mouse fibroblast (3T3) cells cultured in Eagle's medium containing a low concentration of TGF-beta 1 (0.04-1 ng/ml) secreted 3-5 times more LGF than the cells cultured in the absence of TGF-beta 1. The amount of LGF secretion was dose-dependent on the concentration of post-cultured medium and time-dependent after the addition of TGF-beta 1. Similar findings were obtained in human diploid fibroblasts, WI-38 cells. LGF is a 18KD glycoprotein that is acid-stable but heat-unstable.
MeSH terms
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3T3 Cells
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Animals
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Cell Division / drug effects
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Cell Line
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Chromatography, Affinity
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Chromatography, Gel
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Cycloheximide / pharmacology
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Growth Substances / biosynthesis*
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Growth Substances / isolation & purification
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Growth Substances / pharmacology
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Humans
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Intercellular Signaling Peptides and Proteins*
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Kinetics
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Lung
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Mice
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Transforming Growth Factor beta / pharmacology*
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Tumor Cells, Cultured
Substances
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Transforming Growth Factor beta
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leukemia growth-promoting factor
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Cycloheximide