A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing

PCR Methods Appl. 1993 May;2(4):356-8. doi: 10.1101/gr.2.4.356.
No abstract available

MeSH terms

  • Chelating Agents
  • DNA / blood
  • DNA / genetics
  • Evaluation Studies as Topic
  • Gene Amplification
  • Genes, MHC Class II*
  • Heparin / isolation & purification
  • Heparin / pharmacology
  • Humans
  • In Vitro Techniques
  • Nucleic Acid Synthesis Inhibitors
  • Polymerase Chain Reaction / methods*
  • Resins, Synthetic
  • Taq Polymerase

Substances

  • Chelating Agents
  • Nucleic Acid Synthesis Inhibitors
  • Resins, Synthetic
  • Chelex 100
  • Heparin
  • DNA
  • Taq Polymerase