Analysis of the murine All-1 gene reveals conserved domains with human ALL-1 and identifies a motif shared with DNA methyltransferases

Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6350-4. doi: 10.1073/pnas.90.13.6350.

Abstract

A series of translocation break points found in a subset of human acute leukemias have one of the breaks on human chromosome 11q23. This region has recently been cloned and a large gene, ALL-1, with homology to the Drosophila trithorax gene has been identified. This paper describes the cloning, sequencing, and mapping of the mouse homolog of ALL-1. We have found a motif present in All-1 that shows homology to the zinc-binding domain of DNA (cytosine-5) methyltransferases (EC 2.1.1.63). Sequence analysis of the murine All-1 gene has identified distinct regions of homology with the human ALL-1 gene; these highly conserved domains may define regions of functional significance in mammals. In addition, we have identified alternatively spliced forms of All-1 within one of the zinc-finger domains, suggesting that there may be different targets and/or functions for All-1 proteins. Finally, we report that All-1 resides in the proximal portion of mouse chromosome 9 and is a candidate for a mutation that results in skeletal transformations during embryonic development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Conserved Sequence*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Histone-Lysine N-Methyltransferase
  • Humans
  • Methyltransferases / chemistry*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Myeloid-Lymphoid Leukemia Protein
  • O(6)-Methylguanine-DNA Methyltransferase
  • Oncogenes*
  • Open Reading Frames
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Proto-Oncogenes*
  • Transcription Factors*
  • Zinc Fingers

Substances

  • DNA-Binding Proteins
  • KMT2A protein, human
  • Transcription Factors
  • Myeloid-Lymphoid Leukemia Protein
  • Methyltransferases
  • Histone-Lysine N-Methyltransferase
  • Kmt2a protein, mouse
  • O(6)-Methylguanine-DNA Methyltransferase

Associated data

  • GENBANK/L17069