Abstract
The candidate proto-oncogene bcl-3 encodes a protein that shares structural features with I kappa B-alpha and other proteins that bind to members of the Rel protein family. Here, we show that in contrast to the inhibitory activity of I kappa B-alpha, the bcl-3 gene product superactivates NF-kappa B p50 homodimer-mediated gene expression both in vivo and in vitro. BCL-3 protein can, as well, selectively associate with p50 homodimers in the presence of DNA containing a kappa B motif. These results strongly suggest that BCL-3 can act as a transcriptional coactivator, acting through DNA-bound p50 homodimers.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Cell Lymphoma 3 Protein
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Cells, Cultured
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DNA / metabolism
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Gene Expression
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Humans
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Luciferases / genetics
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Mice
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NF-kappa B / metabolism*
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / metabolism
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Trans-Activators*
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Transcription Factors
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Transcription, Genetic*
Substances
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B-Cell Lymphoma 3 Protein
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BCL3 protein, human
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Bcl3 protein, mouse
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MAS1 protein, human
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NF-kappa B
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Trans-Activators
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Transcription Factors
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DNA
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Luciferases