Abstract
cDNAs representing the alpha subunit of polyomavirus enhancer binding protein 2 (PEBP2; also called PEA2) were isolated. The products of the cDNAs are highly homologous to that of Drosophila segmentation gene runt (run) for an N-proximal 128-amino acid region showing 66% identity. The run homology region encompasses the domain capable of binding to a specific nucleotide sequence motif and of dimerizing with the companion beta subunit. The human AML1 gene related to t(8;21) acute myeloid leukemia also had a run homology region. Together with the beta subunit, which increases the affinity of the alpha subunit to DNA without binding to DNA by itself, PEBP2 represents a newly discovered family of transcription factor. The major species of PEBP2 alpha mRNA was expressed in T-cell lines but not in B-cell lines tested. Evidence indicated that PEBP2 functions as a transcriptional activator and is involved in regulation of T-cell-specific gene expression.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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Core Binding Factor Alpha 2 Subunit
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Core Binding Factor alpha Subunits
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DNA-Binding Proteins / genetics*
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Drosophila / genetics
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Drosophila Proteins
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Mice
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Molecular Sequence Data
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Multigene Family / genetics*
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Neoplasm Proteins / genetics*
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Nuclear Proteins
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Proto-Oncogene Proteins*
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RNA, Messenger / genetics
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Regulatory Sequences, Nucleic Acid
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Sequence Analysis
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Sequence Homology, Amino Acid
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Transcription Factor AP-2
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
Substances
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Core Binding Factor Alpha 2 Subunit
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Core Binding Factor alpha Subunits
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DNA-Binding Proteins
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Drosophila Proteins
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Neoplasm Proteins
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Nuclear Proteins
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Proto-Oncogene Proteins
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RNA, Messenger
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Runx1 protein, mouse
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TfAP-2 protein, Drosophila
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Transcription Factor AP-2
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Transcription Factors
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run protein, Drosophila
Associated data
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GENBANK/D14636
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GENBANK/D14637