Molecular cloning and developmental expression of human cardiac troponin T

FEBS Lett. 1993 Aug 9;328(1-2):139-44. doi: 10.1016/0014-5793(93)80981-y.

Abstract

We have isolated a full-size cDNA coding for cardiac troponin T (cTnT) from a human adult heart library, using a slow skeletal TnT probe. This cDNA detected a 1.2 kb mRNA in fetal and post-natal human heart, the amount of which increased during ontogenic development. Interestingly, a similar transcript was coexpressed in fetal skeletal muscle, together with the 0.9 kb slow skeletal muscle mRNA, and its expression was down-regulated during further development.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Child
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Probes
  • Fetal Heart / metabolism
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Myocardium / metabolism*
  • RNA, Messenger / metabolism
  • Rabbits
  • Rats
  • Sheep
  • Troponin / genetics*
  • Troponin / metabolism*
  • Troponin T

Substances

  • DNA Probes
  • RNA, Messenger
  • Troponin
  • Troponin T
  • DNA