Effects of cyclosporin A and verapamil on mouse pancreatic islets

Acta Endocrinol (Copenh). 1993 Jul;129(1):54-8. doi: 10.1530/acta.0.1290054.

Abstract

Treatment of NMRI mice with cyclosporin A (25 mg/kg body wt) for 11 days caused a marked fall in pancreas insulin content, although plasma glucose and plasma insulin were unchanged. When islets from untreated mice were exposed to cyclosporin A (2 mg/l) in vitro, no effect was seen in the first hour. After 24 h, cyclosporin A had significantly decreased the islet content of insulin. Post-culture microperifusion showed that cyclosporin A for 24 or 72 h inhibited the insulin secretory responsiveness. Verapamil in vivo (0.4 mg/kg body wt per day) or in vitro (37.5 micrograms/l) did not modify these effects. Verapamil at 25 mg/l suppressed the release of insulin but afforded no obvious protection against cyclosporin A during culture. The beneficial action of verapamil on islets transplanted to the kidney may reflect renal events rather than a primary interaction of drugs in the islets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cyclosporine / pharmacology*
  • Glucose / pharmacology
  • In Vitro Techniques
  • Insulin / metabolism
  • Insulin Antagonists / pharmacology
  • Islets of Langerhans / drug effects*
  • Islets of Langerhans / metabolism
  • Male
  • Mice
  • Mice, Inbred Strains
  • Verapamil / pharmacology*

Substances

  • Insulin
  • Insulin Antagonists
  • Cyclosporine
  • Verapamil
  • Glucose