Abstract
(22R)-20 alpha,22-Dihydroxycholesterol is the second intermediate in the conversion of cholesterol to pregnenolone by cytochrome P450scc in steroidogenic tissues. We report a rapid method for the enzymatic synthesis of (22R)-20 alpha,22-dihydroxycholesterol from (22R)-22-hydroxycholesterol using mitochondria from the human placenta.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cholesterol / metabolism
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Cholesterol Side-Chain Cleavage Enzyme / metabolism*
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Female
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Humans
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Hydrogen-Ion Concentration
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Hydroxycholesterols / metabolism*
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Kinetics
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Mitochondria / enzymology
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Placenta / enzymology*
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Placenta / ultrastructure
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Pregnancy
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Pregnenolone / metabolism
Substances
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Hydroxycholesterols
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20,22-dihydroxycholesterol
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Pregnenolone
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Cholesterol
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Cholesterol Side-Chain Cleavage Enzyme