Nitrendipine modulates the sodium binding site of cardiac (Na,K)-ATPase

Life Sci. 1993;52(14):1175-9. doi: 10.1016/0024-3205(93)90099-o.

Abstract

The present study deals with the effect of calcium entry blocking agent, nitrendipine, on the rat heart sarcolemmal (Na,K)-ATPase. Nitrendipine (1 mumol/l) exerted a stimulatory effect on (Na,K)-ATPase activity. Kinetic analysis of enzyme activation by increasing concentrations of sodium in the presence of nitrendipine revealed a 67% increase in Vmax value and a decrease of the apparent Ka value to 33% of that in control. The observed influence on (Na,K)-ATPase may contribute indirectly to the calcium antagonistic effect of nitrendipine.

MeSH terms

  • Animals
  • Binding Sites / drug effects
  • In Vitro Techniques
  • Myocardium / cytology
  • Myocardium / enzymology*
  • Nitrendipine / pharmacology*
  • Rats
  • Sarcolemma / drug effects*
  • Sarcolemma / enzymology
  • Sodium / metabolism*
  • Sodium-Potassium-Exchanging ATPase / drug effects*
  • Sodium-Potassium-Exchanging ATPase / metabolism

Substances

  • Nitrendipine
  • Sodium
  • Sodium-Potassium-Exchanging ATPase