Construction of a retrotransposition indicator sequence using a neomycin resistance-encoding gene containing a functional intron

Gene. 1993 May 30;127(2):233-6. doi: 10.1016/0378-1119(93)90725-i.

Abstract

An intron from a Syrian hamster gene was inserted into a neo gene such that splicing of the neo gene mRNA results in the synthesis of active aminoglycoside phosphotransferase. The unspliced construct is inactive in Escherichia coli, but confers resistance to G418 when transfected into mouse and hamster cells. This selectable marker is designed to aid in the cloning and identification of genomic integration sites following retrotransposition.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cricetinae
  • DNA Transposable Elements*
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Gentamicins / pharmacology
  • Introns*
  • Kanamycin Kinase
  • Mesocricetus
  • Mice
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Phosphotransferases / genetics*
  • Polymerase Chain Reaction
  • RNA Splicing
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Restriction Mapping
  • Retroviridae / genetics*
  • Transformation, Bacterial

Substances

  • DNA Transposable Elements
  • Gentamicins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • antibiotic G 418
  • Phosphotransferases
  • Kanamycin Kinase

Associated data

  • GENBANK/L03653
  • GENBANK/L03654
  • GENBANK/L07632
  • GENBANK/L09601
  • GENBANK/L09602
  • GENBANK/X54209
  • GENBANK/X67280
  • GENBANK/X67281
  • GENBANK/X67282
  • GENBANK/X67283