Abstract
Progressive multifocal leukoencephalopathy results from an opportunistic infection of myelin-producing oligodendrocytes by the glia-specific human papovavirus JC. In this report, evidence is presented that the glial transcription factor Tst-1, a member of the POU-domain family, stimulates transcription of both early and late viral genes. Stimulation was dependent on site-specific binding of Tst-1 to the JC viral regulatory region and on the presence of an intact amino-terminal transactivation domain within Tst-1. Because of its ability to increase the expression of viral large tumor antigen, Tst-1 stimulated viral DNA replication, without participating directly in the replication event. Our results suggest that Tst-1 is one of the determining factors in the glia specificity of JC virus.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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DNA-Binding Proteins / genetics*
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Gene Expression Regulation, Viral*
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Genes, Viral
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Humans
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In Vitro Techniques
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JC Virus / genetics*
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JC Virus / growth & development
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Leukoencephalopathy, Progressive Multifocal / genetics
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics
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Octamer Transcription Factor-6
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Oligodeoxyribonucleotides / chemistry
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Transcription Factors / genetics*
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Transcription, Genetic
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Tumor Cells, Cultured
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Viral Proteins / genetics
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Viral Structural Proteins / genetics
Substances
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DNA-Binding Proteins
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Nerve Tissue Proteins
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Oligodeoxyribonucleotides
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POU3F1 protein, human
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Transcription Factors
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Viral Proteins
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Viral Structural Proteins
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Octamer Transcription Factor-6