Prevalence of HPV in a Melbourne female STD population: comparison of RNA and DNA probes in detecting HPV by dot blot hybridization

Int J STD AIDS. 1993 May-Jun;4(3):159-64. doi: 10.1177/095646249300400307.

Abstract

A total of 377 women, consecutively selected as first attenders to a sexually transmitted diseases clinic in Melbourne, Australia, were examined for overt Condylomata acuminata and were screened for genital HPV DNA types 6, 11, 16, 18, 31, 33 and (35) using 2 dot blot hybridization methods. Overall, there was a 90% positivity correlation between the 2 methods with HPV DNA being detected in 12% of ectocervical samples. Overt warts were found in 15% of the women and HPV DNA was detected at the cervix in 35% with cytology predicting HPV with or without dysplasia in 27%. Thirteen percent had a past history of warts but none on examination and HPV DNA was evident in 16% while 18% had cytological features of HPV. Those with no warts evident and no past history of warts had both HPV DNA and cytological features of HPV in 7%.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Condylomata Acuminata / diagnosis
  • Condylomata Acuminata / epidemiology*
  • DNA Probes, HPV* / genetics
  • DNA, Viral / isolation & purification
  • Female
  • Genital Diseases, Female / diagnosis
  • Genital Diseases, Female / epidemiology*
  • Humans
  • Immunoblotting
  • Mass Screening
  • Nucleic Acid Hybridization
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • RNA Probes*
  • Sensitivity and Specificity
  • Tumor Virus Infections / diagnosis
  • Tumor Virus Infections / epidemiology*
  • Victoria / epidemiology

Substances

  • DNA Probes, HPV
  • DNA, Viral
  • RNA Probes