Abstract
False positivity is reported of in situ PCR reactions in a direct incorporation assay with digoxigenin-labelled dUTP. It is recommended that in situ hybridization with specific labelled probe replaces the direct incorporation method for the detection of in situ PCR amplicon.
MeSH terms
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Apoptosis
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Biopsy
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Cells, Cultured
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DNA
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DNA, Viral / analysis
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DNA-Directed DNA Polymerase
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Deoxyuracil Nucleotides
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Digoxigenin
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False Positive Reactions
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Fibroblasts / cytology
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Herpes Simplex / diagnosis
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Humans
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In Situ Hybridization / methods*
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Lymph Nodes / pathology*
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Polymerase Chain Reaction / methods*
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Protein-Tyrosine Kinases / analysis
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Proto-Oncogene Proteins / analysis
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Proto-Oncogene Proteins c-bcl-2
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Simplexvirus / genetics
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Simplexvirus / isolation & purification
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Taq Polymerase
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Translocation, Genetic
Substances
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DNA, Viral
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Deoxyuracil Nucleotides
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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deoxyuridine triphosphate
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DNA
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Protein-Tyrosine Kinases
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Taq Polymerase
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DNA-Directed DNA Polymerase
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Digoxigenin