Abstract
Human papilloma virus type 16 (HPV16) related sequences were detected in two EBV-negative nasopharyngeal carcinoma (NPC) cell lines derived from two well-differentiated NPC by polymerase chain reaction. E2 and E6 related sequences of HPV16 were demonstrated using two pairs of primers derived from these two regions. DNA sequence analysis of amplified products excluded the possibility of laboratory viral DNA contamination.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, Viral / genetics
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Base Sequence
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Cell Differentiation
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DNA Probes, HPV
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DNA, Viral / genetics*
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DNA, Viral / isolation & purification
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / isolation & purification
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Humans
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Molecular Sequence Data
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Nasopharyngeal Neoplasms / microbiology*
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Nasopharyngeal Neoplasms / pathology
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Papillomaviridae / genetics*
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Papillomaviridae / isolation & purification
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Polymerase Chain Reaction
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Tumor Cells, Cultured / microbiology
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Tumor Cells, Cultured / pathology
Substances
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Antigens, Viral
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DNA Probes, HPV
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DNA, Viral