PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants

P M Kroisel; P A Ioannou; P J de Jong

doi:10.1159/000133609

PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants

Cytogenet Cell Genet. 1994;65(1-2):97-100. doi: 10.1159/000133609.

Authors

P M Kroisel¹, P A Ioannou, P J de Jong

Affiliation

¹ Human Genome Center, Lawrence Livermore National Laboratory, University of California, Livermore.

PMID: 8404074
DOI: 10.1159/000133609

Abstract

We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cloning, Molecular
DNA Probes
DNA, Bacterial / analysis*
DNA, Recombinant / analysis*
Genetic Vectors
In Situ Hybridization, Fluorescence*
Polymerase Chain Reaction / methods*

Substances

DNA Probes
DNA, Bacterial
DNA, Recombinant