Fragmentation of DNA in P388D1 macrophages exposed to oxidised low-density lipoprotein

FEBS Lett. 1993 Oct 18;332(3):218-20. doi: 10.1016/0014-5793(93)80635-8.

Abstract

Exposing a macrophage-like murine cell line to copper-oxidised low-density lipoprotein led to DNA fragmentation which was inhibited by the putative Ca2+/Mg2+ endonuclease inhibitor, zinc sulphate. DNA fragmentation preceded loss of membrane impermeability. These results suggest that apoptosis may be a mechanism of macrophage foam cell death in atherosclerotic lesions in the arterial wall.

MeSH terms

  • Adenine Nucleotides / metabolism
  • Animals
  • Cell Line
  • DNA / biosynthesis
  • DNA / isolation & purification
  • DNA / metabolism*
  • DNA Damage*
  • Electrophoresis, Agar Gel
  • Humans
  • Kinetics
  • Lipoproteins, LDL / blood
  • Lipoproteins, LDL / isolation & purification
  • Lipoproteins, LDL / toxicity*
  • Macrophages / drug effects
  • Macrophages / metabolism*

Substances

  • Adenine Nucleotides
  • Lipoproteins, LDL
  • DNA