Abstract
The structural gene encoding 25-hydroxyvitamin D3 24-hydroxylase (P-450cc24) was isolated from the rat genomic DNA. It spans approximately 15 kb, is composed of 12 exons, and was demonstrated by Southern blot analysis to be present as a single copy. One major T residue was identified at the cap site, a putative TATA (ATAAATA) box was located at position -30, and a putative CCAAT box was at -58. Four possible vitamin D responsive elements that may be involved in regulation of 24-hydroxylase expression were found in the 5'-flanking region. Alignment with mitochondrial P-450 proteins showed that 7 out of 11 intron insertion sites of P-450cc24 gene occupied positions identical with those in the CYP11 family (P-450scc, P-450(11 beta)). The structure of the gene is discussed in relation to present knowledge about the mechanism of regulation of the 25-hydroxyvitamin D3 24-hydroxylase and calcium homeostasis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Cholesterol Side-Chain Cleavage Enzyme / genetics
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Cytochrome P-450 CYP11B2
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Cytochrome P-450 Enzyme System / chemistry
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Cytochrome P-450 Enzyme System / genetics*
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DNA / chemistry*
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Exons
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Genes
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Introns
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Mitochondria / enzymology
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Molecular Sequence Data
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Rats
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Rats, Wistar
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Sequence Homology, Amino Acid
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Steroid Hydroxylases / chemistry
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Steroid Hydroxylases / genetics*
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Transcription, Genetic
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Vitamin D3 24-Hydroxylase
Substances
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DNA
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Cytochrome P-450 Enzyme System
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Steroid Hydroxylases
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Vitamin D3 24-Hydroxylase
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Cytochrome P-450 CYP11B2
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Cholesterol Side-Chain Cleavage Enzyme
Associated data
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GENBANK/D17792
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GENBANK/L04608
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GENBANK/L04609
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GENBANK/L04610
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GENBANK/L04611
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GENBANK/L04612
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GENBANK/L04613
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GENBANK/L04614
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GENBANK/L04615
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GENBANK/L04616
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GENBANK/L04617
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GENBANK/L04618
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GENBANK/L04619